biotinylated horse antigoat secondary antibody (Vector Laboratories)

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Vector Laboratories biotinylated horse antigoat secondary antibody
Biotinylated Horse Antigoat Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1679 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+horse+antigoat/Biotinylated+Horse+Anti-Goat+IgG+Antibody/pmc12856726-92-26-32
Average 96 stars, based on 1679 article reviews

biotinylated horse antigoat secondary antibody - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: Endothelial CB1-receptors limit infarct size through NO formation in rat isolated hearts.
Article Snippet: The aim of the present study was to document the presence of cannabinoid receptors in the rat heart, and to assess the cardioprotective properties of CB1and CB2-receptor agonists.. Rat isolated hearts were exposed to low-flow ischemia and reperfusion, with selective cannabinoid agonists administered prior to and during the ischemic period.. In some hearts, RT-PCR, Western blots, and immunohistological techniques were used to identify and localize both cannabinoid-receptor subtypes.

Article Title: Time-Dependent Alterations in the Expression of NMDA Receptor Subunits along the Dorsoventral Hippocampal Axis in an Animal Model of Nascent Psychosis.
Article Snippet: Psychosis is a mental condition that is characterized by hallucinations, delusions, disordered thought, as well as socio-emotional and cognitive impairments.. Once developed, it tends to progress into a chronic psychotic illness.. Here, the duration of untreated psychosis plays a crucial role: the earlier the treatment begins, relative to the first episode of the disease, the better the patient’s functional prognosis.

Article Title: Thrombin and interleukin-1beta regulate HOXA10 expression in human term decidual cells: implications for preterm labor.
Article Snippet: .. Biotinylated horse antigoat (Vector Laboratories, Burlingame, CA) was applied at 3.5 g/ml for 1 h at 4 C. Slides were washed in 1 PBST, incubated in ABC Elite (Vector) for 15 min at room temperature, washed in 1 PBST, and incubated for 5 min in diaminobenzidine (Vector). ..

Article Title: Prenatal choline supplementation attenuates neuropathological response to status epilepticus in the adult rat hippocampus
Article Snippet: Free-floating sections were rinsed in TBS, treated for 30 minutes in 50% methanol, treated for 30 minutes in 0.6% hydrogen peroxide, incubated in 0.1% TTX and 3% normal horse serum in TBS for 30 minutes at room temperature, and then incubated with the primary antibody (affinity purified polyclonal goat antibody raised against a peptide mapping at the carboxy terminus of human DCX, 1:200; Santa Cruz Biotechnology, Santa Cruz, CA) for 4 hours at room temperature. .. Following this, the tissue was rinsed with TBS and incubated with the secondary antibody (biotinylated horse antigoat, 1:200; Vector Laboratories) for 2 hours at room temperature. .. The tissue was then rinsed in TBS, incubated in an avidin-biotinylated peroxidase complex (ABC, Vector Laboratories) for 1 hour at room temperature, rinsed again in TBS, and developed with vector grey substrate (Vector Laboratories).

Immunohistochemistry:

Article Title: Socs2 and elf5 mediate prolactin-induced mammary gland development.
Article Snippet: .. Elf5 immunohistochemistry was performed using the Elf-5 (N-20) affinity-purified goat polyclonal antibody (sc-9645) from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA) at a dilution of 1:300, and a secondary biotinylated horse antigoat (Vector Laboratories, Inc., Burlingame, CA), LSAB (DAKO) and was detected using Liquid DAB Substrate Chromogen (DAKO). .. The antimilk (1:12,000) primary antibody (Accurate Chemical & Scientific Corp., Westbury, NY) was incubated for 30 min, and bound antibody was detected using the Envision System (DAKO) and 3,3 -diaminobenzidine Plus (DAKO) as substrate.

Article Title: Growth differentiation factor 9 is a germ cell regulator of Sertoli cell function.
Article Snippet: The following antibodies were purchased commercially: goat antihuman GDF9 (catalog no. sc-12244) and rabbit antihuman BMP15 (GDF9B catalog no. sc-28911) from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA); rabbit antihuman BMPR1B (referred to as ALK6; catalog no. AP2005b) from Abgent (San Diego, CA); goat antihuman BMPRII (catalog no. AF811) from R&D Systems, Inc. (Minneapolis, MN); rabbit anti-occludin and rabbit anti-claudin-11 from Zymed Laboratories, Inc. (San Francisco, CA); and rabbit anti- -catenin and zonula occludens-1 (ZO-1) (Sigma Chemical). .. Primary antibodies were detected with various secondary antibodies as follows: Alexa Fluor 488-goat antirabbit IgG and Alexa Fluor 488-rabbit antigoat IgG from Molecular Probes, Inc. (Eugene, OR), used in conjunction with the nuclear counterstain TOPRO-3 (Molecular Probes) for immunofluorescence, and biotinylated horse antigoat from Vector Laboratories (Burlingame, CA) and sheep antirabbit from CHEMICON International, Inc. (Temecula, CA), together with Vectastain ABC amplification (Vector Laboratories) for conventional immunohistochemistry. .. Negative controls were obtained as follows: rabbit IgG from Dako Corp. (Carpinteria, CA) and goat IgG from Serotec (Oxford, UK).

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biotinylated horse antigoat secondary antibody (Vector Laboratories)

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